ABSTRACT
Association between mangrove plants with fungi has been explored for many biological activities. The purpose of thisstudy was to determine the antimicrobial and cytotoxic activities of endophytic fungi isolated from mangrove plantsScyphiphora hydrophyllacea C. F. Gaertn. The isolation of endophytic fungus from the mangrove has been carriedout by the direct planting method on Sabouraud Dextrose Agar medium. Eleven fungal isolates were recovered andpurified. The isolates were cultivated on the rice medium for 1 month and extracted using ethyl acetate. Eleven ethylacetate extracts were screened for cytotoxic activity by Brine Shrimp Lethality Test method and 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide assay and for antimicrobial activity toward some human pathogenic microbes.The results showed that five (45.5%) isolates of endophytic fungi had antimicrobial activity against the growth ofStaphylococcus aureus, Escherichia coli, and Candida albicans with a diameter from 9.1 to 21 mm. Furthermore, fourextracts (36.4%) were cytotoxic (cell viability < 50%) against T47D cells. Isolate ADSH2 which exhibited the highestcytotoxic activity was identified molecularly as Penicillium chrysogenum. The results of this study indicate that theethyl acetate of endophytic fungi associated with Scyphiphora hydrophyllacea has the potential to be developed asantimicrobial and anticancer compounds.
ABSTRACT
This study aims to evaluate the antimicrobial activities of endophytic fungi from marine brown algae Padina sp.collected from Nirwana Beach, Padang, West Sumatera, Indonesia. The isolation of endophytic fungi was conductedusing dilution method with Sabouraud Dextrose Agar (SDA) + Chloramphenicol as a growth medium. Nine fungistrains have been isolated from this alga. Purely isolated fungi were cultivated using rice as a medium at roomtemperature for 3–4 weeks. The secondary metabolite produced by fungi was extracted using ethyl acetate (EtOAc)as a solvent. The antimicrobial activity of EtOAc extracts was tested against Staphylococcus aureus, Escherichiacoli, and Candida albicans by using the agar diffusion method. In this research, nine endophytic fungi were isolatedfrom the brown marine algae Padina sp. The results of antimicrobial activity screening showed that one fungal isolate(Nita3) was selected as the most active against S. aureus, E. coli, and C. albicans with a diameter of inhibition zone of20.98 ± 1.56 mm, 17.98 ± 6.58 mm, and 13.60 ± 0 mm, respectively. This selected fungus was identified molecularlyas Trichoderma harzianum. We conclude that T. harzianum can be a source of antimicrobial compounds. However,continuous research is needed to prove its bioactive action
ABSTRACT
This study aims to evaluate the antimicrobial activities of endophytic fungi from marine brown algae Padina sp.collected from Nirwana Beach, Padang, West Sumatera, Indonesia. The isolation of endophytic fungi was conductedusing dilution method with Sabouraud Dextrose Agar (SDA) + Chloramphenicol as a growth medium. Nine fungistrains have been isolated from this alga. Purely isolated fungi were cultivated using rice as a medium at roomtemperature for 3–4 weeks. The secondary metabolite produced by fungi was extracted using ethyl acetate (EtOAc)as a solvent. The antimicrobial activity of EtOAc extracts was tested against Staphylococcus aureus, Escherichiacoli, and Candida albicans by using the agar diffusion method. In this research, nine endophytic fungi were isolatedfrom the brown marine algae Padina sp. The results of antimicrobial activity screening showed that one fungal isolate(Nita3) was selected as the most active against S. aureus, E. coli, and C. albicans with a diameter of inhibition zone of20.98 ± 1.56 mm, 17.98 ± 6.58 mm, and 13.60 ± 0 mm, respectively. This selected fungus was identified molecularlyas Trichoderma harzianum. We conclude that T. harzianum can be a source of antimicrobial compounds. However,continuous research is needed to prove its bioactive action.